Composite

Part:BBa_K3826009:Design

Designed by: Lucas Müller   Group: iGEM21_Duesseldorf   (2021-10-21)


p35s RUBY HSP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4600
    Illegal BamHI site found at 3249
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1185
    Illegal NgoMIV site found at 1764
    Illegal NgoMIV site found at 3477
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 66


Design Notes

The 35S promoter is a strong promoter derived from cauliflower mosaic virus. BBa_K1537015 RUBY as commonly used reporter, (Yubing He et al. 2020), BBa_K3900028 HSP: Terminator in Arabidopsis thaliana (S Nagaya et al,2009)



Source

The 35S promoter is a strong promoter derived from cauliflower mosaic virus. BBa_K1537015 RUBY as commonly used reporter, (Yubing He et al. 2020), BBa_K3900028 HSP: Terminator in Arabidopsis thaliana (S Nagaya et al,2009)


References